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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
24/06/2019 |
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Data da última atualização: |
24/06/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PERON, F. N.; CALLONI, R.; VENTURA, J. A.; FERNANDES, P. M. B. |
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Afiliação: |
UFES; UFGRS; Jose Aires Ventura, Incaper; UFES. |
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Título: |
Bioinformatics approach to the study of the molecular behaviour of mealybug wilt of pineapple. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Horticulturae, 1239, p. 177-184, 2019. |
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DOI: |
10.17660/ActaHortic.2019.1239.22 |
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Idioma: |
Inglês |
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Conteúdo: |
Mealybug wilt of pineapple (MWP) is a disease caused by the Pineapple mealybug wilt-associated virus (PMWaV) complex transmitted by Dysmicoccus brevipes and D. neobrevipes. MWP symptoms are characterized by root dessication, leaf wilting and consequent failure to produce a fruit. The molecular mechanisms involved in the pineapple-PMWaV interaction for MWP symptomatology are still unclear. In this work, mRNAs of asymptomatic and symptomatic pineapple plants were evaluated using Illumina RNA sequencing technology. From a total of 79 million reads per sample, 16,097 genes were identified using STAR aligner and HTseq for paired-end files. Differentially expressed genes (DEGs) between the evaluated groups were estimated using DESeq2 and edgeR, with an FDR cutoff of ≤0.05. A total of 207 DEGs were detected, with 61 upregulated and 146 downregulated in symptomatic plants infected by PMWaV-2. The methodologies improved by the assays presented in this article and the detected DEGs can substantiate further researches with pineapple and the MWP disease. |
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Palavras-Chave: |
Abacaxi. |
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Thesaurus NAL: |
Bioinformatics; DESeq2; EdgeR; Pineapple; PMWaV; STAR; Transcriptomic. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 1021431
005 2019-06-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.17660/ActaHortic.2019.1239.22$2DOI
100 1 $aPERON, F. N.
245 $aBioinformatics approach to the study of the molecular behaviour of mealybug wilt of pineapple.$h[electronic resource]
260 $c2019
520 $aMealybug wilt of pineapple (MWP) is a disease caused by the Pineapple mealybug wilt-associated virus (PMWaV) complex transmitted by Dysmicoccus brevipes and D. neobrevipes. MWP symptoms are characterized by root dessication, leaf wilting and consequent failure to produce a fruit. The molecular mechanisms involved in the pineapple-PMWaV interaction for MWP symptomatology are still unclear. In this work, mRNAs of asymptomatic and symptomatic pineapple plants were evaluated using Illumina RNA sequencing technology. From a total of 79 million reads per sample, 16,097 genes were identified using STAR aligner and HTseq for paired-end files. Differentially expressed genes (DEGs) between the evaluated groups were estimated using DESeq2 and edgeR, with an FDR cutoff of ≤0.05. A total of 207 DEGs were detected, with 61 upregulated and 146 downregulated in symptomatic plants infected by PMWaV-2. The methodologies improved by the assays presented in this article and the detected DEGs can substantiate further researches with pineapple and the MWP disease.
650 $aBioinformatics
650 $aDESeq2
650 $aEdgeR
650 $aPineapple
650 $aPMWaV
650 $aSTAR
650 $aTranscriptomic
653 $aAbacaxi
700 1 $aCALLONI, R.
700 1 $aVENTURA, J. A.
700 1 $aFERNANDES, P. M. B.
773 $tActa Horticulturae, 1239, p. 177-184, 2019.
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Biblioteca Rui Tendinha (BRT) |
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| Registros recuperados : 4 | |
| 1. |  | PERON, F. N.; FERNANDES, P. M. B.; VENTURA, J. A. Detecção do pineapple mealybug wilt-associated virus (PMWaV1, PMWaV2 e PMWaV3) em abacaxizeiros no Estado do Espírito Santo. Tropical Plant Pathology, Brasília, DF, v. 35, p. S185, ago. 2010. Suplemento, ref. 05.091. Edição dos resumos do XLIII Congresso Brasileiro de Fitopatologia, Cuiabá, ago. 2010.| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 4. | | PIASSI, M.; VENTURA, J. A.; PIASSI, M.; CAETANO, L. C. S.; COSTA, A. F. da.; DOUSSEAU, S.; PERON, F. N. Novas tecnologias para a propagação in vitro e limpeza clonal de abacaxizeiro. In: CONGRESSO CAPIXABA DE PESQUISA AGROPECUÁRIA, 1., Vitória, ES. Anais 2021 : congresso capixaba de pesquisa agropecuária [recurso eletrônico]. Vitória, ES: Incaper, 2021. color. PDF ; 25,4 MB. E-book, no formato PDF. (Incaper, Documentos, 289). Pedro Luís Pereira Teixeira de Carvalho, Carlos Henrique Rodrigues de Oliveira, José Aires Ventura, Marcos Vinicius Winckler Caldeira e Romário Gava Ferrão, editores. p. 183| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| Registros recuperados : 4 | |
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