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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
26/01/2018 |
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Data da última atualização: |
26/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OGAWA, E. M.; COSTA, H. B.; VENTURA, J. A.; CAETANO, L. C. S.; PINTO, F. E.; OLIVEIRA, B. G.; BARROSO, M. E. S.; SCHERER, R.; ENDRINGER, D. C.; ROMÃO, W. |
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Afiliação: |
UFES; UFES; Jose Aires Ventura, Incaper; Luiz Carlos Santos Caetano, Incaper; UFES; UFES; UVV; UVV; UVV; UFES. |
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Título: |
Chemical profile of pineapple cv. Vitória in different maturation stages using electrospray ionization mass spectrometry. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of the Science of Food and Agriculture, v. 98, n. 3, p. 1105-1116, fev. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
BACKGROUND:
Pineapple is the fruit of Ananas comosus var. comosus plant, being cultivated in tropical areas and has high energy content and nutritional value. Herein, 30 samples of pineapple cv. Vitória were analyzed as a function of the maturation stage (0-5) and their physico-chemical parameters monitored. In addition, negative-ion mode electrospray ionization mass spectrometry [ESI(-)FT-ICR MS] was used to identify and semi-quantify primary and secondary metabolites present in the crude and phenolic extracts of pineapple, respectively.
RESULTS:
Physico-chemical tests show an increase in the total soluble solids (TSS) values and in the TSS/total titratable acidity ratio as a function of the maturity stage, where a maximum value was observed in stage 3 (¾ of the fruit is yellow, which corresponds to the color of the fruit peel). ESI(-)FT-ICR MS analysis for crude extracts showed the presence mainly of sugars as primary metabolites present in deprotonated molecule form ([M?-?H]- and [2?M?-?H]- ions) whereas, for phenolic fractions, 11 compounds were detected, being the most abundant in the third stage of maturation. This behavior was confirmed by quantitative analysis of total polyphenols.
CONCLUSION:
ESI-FT-ICR MS was efficient in identifying primary (carbohydrates and organic acids) and secondary metabolites (13 phenolic compounds) presents in the crude and phenolic extract of the samples, respectively. © 2017 Society of Chemical Industry. |
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Palavras-Chave: |
Abacaxi. |
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Thesaurus NAL: |
Electrospray mass spectrometry; Maturation stages; Pineapple. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02331naa a2200277 a 4500
001 1018146
005 2018-01-26
008 2018 bl uuuu u00u1 u #d
100 1 $aOGAWA, E. M.
245 $aChemical profile of pineapple cv. Vitória in different maturation stages using electrospray ionization mass spectrometry.$h[electronic resource]
260 $c2018
520 $aBACKGROUND: Pineapple is the fruit of Ananas comosus var. comosus plant, being cultivated in tropical areas and has high energy content and nutritional value. Herein, 30 samples of pineapple cv. Vitória were analyzed as a function of the maturation stage (0-5) and their physico-chemical parameters monitored. In addition, negative-ion mode electrospray ionization mass spectrometry [ESI(-)FT-ICR MS] was used to identify and semi-quantify primary and secondary metabolites present in the crude and phenolic extracts of pineapple, respectively. RESULTS: Physico-chemical tests show an increase in the total soluble solids (TSS) values and in the TSS/total titratable acidity ratio as a function of the maturity stage, where a maximum value was observed in stage 3 (¾ of the fruit is yellow, which corresponds to the color of the fruit peel). ESI(-)FT-ICR MS analysis for crude extracts showed the presence mainly of sugars as primary metabolites present in deprotonated molecule form ([M?-?H]- and [2?M?-?H]- ions) whereas, for phenolic fractions, 11 compounds were detected, being the most abundant in the third stage of maturation. This behavior was confirmed by quantitative analysis of total polyphenols. CONCLUSION: ESI-FT-ICR MS was efficient in identifying primary (carbohydrates and organic acids) and secondary metabolites (13 phenolic compounds) presents in the crude and phenolic extract of the samples, respectively. © 2017 Society of Chemical Industry.
650 $aElectrospray mass spectrometry
650 $aMaturation stages
650 $aPineapple
653 $aAbacaxi
700 1 $aCOSTA, H. B.
700 1 $aVENTURA, J. A.
700 1 $aCAETANO, L. C. S.
700 1 $aPINTO, F. E.
700 1 $aOLIVEIRA, B. G.
700 1 $aBARROSO, M. E. S.
700 1 $aSCHERER, R.
700 1 $aENDRINGER, D. C.
700 1 $aROMÃO, W.
773 $tJournal of the Science of Food and Agriculture$gv. 98, n. 3, p. 1105-1116, fev. 2018.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
|
Data corrente: |
12/08/2019 |
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Data da última atualização: |
12/08/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
DE FARIAS VIÉGAS AGUIJE, G. M.; ZORZAL, P. B.; BUSS, D. S.; VENTURA, J. A.; FERNANDES, P. M. B.; FERNANDES, A. A. R. |
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Afiliação: |
Glória Maria de Farias Viégas Aquije, UFES; Poliana Belisário Zorzal, UFES; David Shaun Buss, UFES; Jose Aires Ventura, Incaper; Patricia Machado Bueno Fernandes, UFES; Antonio Alberto Ribeiro Fernandes, UFES. |
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Título: |
Cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 29, n. 10, p. 1109-1117, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Fusariosis, caused by the fungus Fusarium subglutinans f. sp. ananas (Syn. F. guttiforme), is one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). Identification of plant cell responses to pathogens is important in understanding the plant?pathogen relationship and establishing strategies to improve and select resistant cultivars. Studies of the structural properties and phenolic content of cell walls in resistant (Vitoria) and susceptible (Perola) pineapple cultivars, related to resistance to the fungus, were performed. The non-chlorophyll base of physiologically mature leaves was inoculated with a conidia suspension. Analyses were performed post-inoculation by light, atomic force, scanning and transmission electron microscopy, and measurement of cell wall-bound phenolic compounds. Non-inoculated leaves were used as controls to define the constitutive tissue characteristics. Analyses indicated that morphological differences, such as cell wall thickness, cicatrization process and lignification, were related to resistance to the pathogen. Atomic force microscopy indicated a considerable difference in the mechanical properties of the resistant and susceptible cultivars, with more structural integrity, associated with higher levels of cell wall-bound phenolics, found in the resistant cultivar. p-Coumaric and ferulic acids were shown to be the major phenolics bound to the cell walls and were found in higher amounts in the resistant cultivar. Leaves of the resistant cultivar had reduced fungal penetration and a faster and more effective cicatrization response compared to the susceptible cultivar. MenosFusariosis, caused by the fungus Fusarium subglutinans f. sp. ananas (Syn. F. guttiforme), is one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). Identification of plant cell responses to pathogens is important in understanding the plant?pathogen relationship and establishing strategies to improve and select resistant cultivars. Studies of the structural properties and phenolic content of cell walls in resistant (Vitoria) and susceptible (Perola) pineapple cultivars, related to resistance to the fungus, were performed. The non-chlorophyll base of physiologically mature leaves was inoculated with a conidia suspension. Analyses were performed post-inoculation by light, atomic force, scanning and transmission electron microscopy, and measurement of cell wall-bound phenolic compounds. Non-inoculated leaves were used as controls to define the constitutive tissue characteristics. Analyses indicated that morphological differences, such as cell wall thickness, cicatrization process and lignification, were related to resistance to the pathogen. Atomic force microscopy indicated a considerable difference in the mechanical properties of the resistant and susceptible cultivars, with more structural integrity, associated with higher levels of cell wall-bound phenolics, found in the resistant cultivar. p-Coumaric and ferulic acids were shown to be the major phenolics bound to the cell walls and were found in higher amounts in the resistant cultivar. Leaves of... Mostrar Tudo |
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Palavras-Chave: |
Abacaxi; Fusariose; Variedade Perola; Variedade Vitoria. |
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Thesaurus NAL: |
Ananas comosus; Disease; Fungus; Fusarium subglutinans; Parasite interaction; Pineapple; Resistant cultivar. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02552naa a2200313 a 4500
001 1021540
005 2019-08-12
008 2010 bl uuuu u00u1 u #d
100 1 $aDE FARIAS VIÉGAS AGUIJE, G. M.
245 $aCell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars.$h[electronic resource]
260 $c2010
520 $aFusariosis, caused by the fungus Fusarium subglutinans f. sp. ananas (Syn. F. guttiforme), is one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). Identification of plant cell responses to pathogens is important in understanding the plant?pathogen relationship and establishing strategies to improve and select resistant cultivars. Studies of the structural properties and phenolic content of cell walls in resistant (Vitoria) and susceptible (Perola) pineapple cultivars, related to resistance to the fungus, were performed. The non-chlorophyll base of physiologically mature leaves was inoculated with a conidia suspension. Analyses were performed post-inoculation by light, atomic force, scanning and transmission electron microscopy, and measurement of cell wall-bound phenolic compounds. Non-inoculated leaves were used as controls to define the constitutive tissue characteristics. Analyses indicated that morphological differences, such as cell wall thickness, cicatrization process and lignification, were related to resistance to the pathogen. Atomic force microscopy indicated a considerable difference in the mechanical properties of the resistant and susceptible cultivars, with more structural integrity, associated with higher levels of cell wall-bound phenolics, found in the resistant cultivar. p-Coumaric and ferulic acids were shown to be the major phenolics bound to the cell walls and were found in higher amounts in the resistant cultivar. Leaves of the resistant cultivar had reduced fungal penetration and a faster and more effective cicatrization response compared to the susceptible cultivar.
650 $aAnanas comosus
650 $aDisease
650 $aFungus
650 $aFusarium subglutinans
650 $aParasite interaction
650 $aPineapple
650 $aResistant cultivar
653 $aAbacaxi
653 $aFusariose
653 $aVariedade Perola
653 $aVariedade Vitoria
700 1 $aZORZAL, P. B.
700 1 $aBUSS, D. S.
700 1 $aVENTURA, J. A.
700 1 $aFERNANDES, P. M. B.
700 1 $aFERNANDES, A. A. R.
773 $tPlant Cell Reports$gv. 29, n. 10, p. 1109-1117, 2010.
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