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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
14/07/2015 |
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Data da última atualização: |
23/10/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, C.; VENTURA, J. A.; COSTA, H.; FERNANDES, P. M. B.; LIMA, N. |
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Afiliação: |
Cledir Santos; Jose Aires Ventura, Incaper; Helcio Costa, Incaper; Nelson Lima. |
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Título: |
MALDI-TOF MS to identify the pineapple pathogen Fusarium guttiforme and its antagonist Trichoderma asperellum on decayed pineapple. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 40, Issue 4, p. 227-232, august 2015. |
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DOI: |
10.1007/s40858-015-0027-7 |
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Idioma: |
Português |
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Conteúdo: |
Matrix-assisted laser desorption⁄ionisation time-offlight mass spectrometry (MALDI-TOF MS) has been used to identify some Fusarium and Trichoderma species, but early detection of fungal diseases by this technique has not yet been fully addressed. In this study, MALDI-TOF MS was tested to identify F. guttiforme on pineapple side shoots in situ. The efficacy of filamentous fungi for controlling fungal diseases is well documented. However, there is uncertainty whether the biocontrol agent is out growing the pathogen sufficiently to be identified. In this paper, a multistep identification of a plant pathogen (F. guttiforme) and its antagonist (T. asperellum) using MALDI-TOF MS is demonstrated. |
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Thesaurus NAL: |
Fungal early detection; Fungal ribosomal proteins; MALDI-TOFMS; Pineapple. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01445naa a2200229 a 4500
001 1006692
005 2015-10-23
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s40858-015-0027-7$2DOI
100 1 $aSANTOS, C.
245 $aMALDI-TOF MS to identify the pineapple pathogen Fusarium guttiforme and its antagonist Trichoderma asperellum on decayed pineapple.$h[electronic resource]
260 $c2015
520 $aMatrix-assisted laser desorption⁄ionisation time-offlight mass spectrometry (MALDI-TOF MS) has been used to identify some Fusarium and Trichoderma species, but early detection of fungal diseases by this technique has not yet been fully addressed. In this study, MALDI-TOF MS was tested to identify F. guttiforme on pineapple side shoots in situ. The efficacy of filamentous fungi for controlling fungal diseases is well documented. However, there is uncertainty whether the biocontrol agent is out growing the pathogen sufficiently to be identified. In this paper, a multistep identification of a plant pathogen (F. guttiforme) and its antagonist (T. asperellum) using MALDI-TOF MS is demonstrated.
650 $aFungal early detection
650 $aFungal ribosomal proteins
650 $aMALDI-TOFMS
650 $aPineapple
700 1 $aVENTURA, J. A.
700 1 $aCOSTA, H.
700 1 $aFERNANDES, P. M. B.
700 1 $aLIMA, N.
773 $tTropical Plant Pathology$gv. 40, Issue 4, p. 227-232, august 2015.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
24/05/2016 |
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Data da última atualização: |
24/05/2016 |
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Autoria: |
RODRIGUES, S. P.; CUNHA, M. da.; VENTURA, J. A.; FERNANDES, P. M. B. |
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Afiliação: |
UFES; UENF; Jose Aires Ventura, Incaper; UFES. |
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Título: |
Effcts of the Papaya meleira virus on papaya latex structure and composition. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
BIOTIC AND BIOTIC STRESS, v. 28, n. 5, p. 861-871, 2009. |
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DOI: |
10.1007/s00299-009-0673-7 |
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Idioma: |
Português |
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Conteúdo: |
Spontaneous latex exudation is the main symptom of papaya sticky (meleira) disease caused by the Papaya meleira virus (PMeV), a double-stranded RNA (dsRNA) virus. This paper describes different effects of PMeV on papaya latex. Latex samples were subjected to different histochemical tests to evaluate their chemical composition. Additionally, the integrity of the latex particles was assessed by transmission and scanning electron microscopy analysis. Biochemical and micro- and macro-element measurements were performed. PMeV dsRNA extraction was performed to evaluate the interaction of the virus with the latex particles. Sticky diseased latex was positive for alkaloid biosynthesis and showed an accumulation of calcium oxalate crystals. PMeV also increased H(2)O(2) synthesis within sticky diseased laticifers. The protein, sugar and water levels were altered, probably due to chemical changes. The morphology of the latex particles was further altered; PMeV particles seemed to be bound to the latex particles. The alkaloid and H(2)O(2) biosynthesis in the papaya laticifers indicate a papaya defense response against PMeV. However, such efforts failed, as the virus affected the plant latex. The effects described here suggest some advantages of the infection process, including facilitating the movement of the virus within the papaya plant. |
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Palavras-Chave: |
Mamão; Papaya. |
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Thesaurus NAL: |
Carica papaya; Latex; Laticifers; Meleira; Papaya meleira virus; Papaya sticky disease. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02100naa a2200265 a 4500
001 1010909
005 2016-05-24
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1007/s00299-009-0673-7$2DOI
100 1 $aRODRIGUES, S. P.
245 $aEffcts of the Papaya meleira virus on papaya latex structure and composition.$h[electronic resource]
260 $c2009
520 $aSpontaneous latex exudation is the main symptom of papaya sticky (meleira) disease caused by the Papaya meleira virus (PMeV), a double-stranded RNA (dsRNA) virus. This paper describes different effects of PMeV on papaya latex. Latex samples were subjected to different histochemical tests to evaluate their chemical composition. Additionally, the integrity of the latex particles was assessed by transmission and scanning electron microscopy analysis. Biochemical and micro- and macro-element measurements were performed. PMeV dsRNA extraction was performed to evaluate the interaction of the virus with the latex particles. Sticky diseased latex was positive for alkaloid biosynthesis and showed an accumulation of calcium oxalate crystals. PMeV also increased H(2)O(2) synthesis within sticky diseased laticifers. The protein, sugar and water levels were altered, probably due to chemical changes. The morphology of the latex particles was further altered; PMeV particles seemed to be bound to the latex particles. The alkaloid and H(2)O(2) biosynthesis in the papaya laticifers indicate a papaya defense response against PMeV. However, such efforts failed, as the virus affected the plant latex. The effects described here suggest some advantages of the infection process, including facilitating the movement of the virus within the papaya plant.
650 $aCarica papaya
650 $aLatex
650 $aLaticifers
650 $aMeleira
650 $aPapaya meleira virus
650 $aPapaya sticky disease
653 $aMamão
653 $aPapaya
700 1 $aCUNHA, M. da.
700 1 $aVENTURA, J. A.
700 1 $aFERNANDES, P. M. B.
773 $tBIOTIC AND BIOTIC STRESS$gv. 28, n. 5, p. 861-871, 2009.
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