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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
23/02/2023 |
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Data da última atualização: |
25/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAURASTONI, M.; ANTUNES, T. F. S.; ABREU, E. F. M.; RIBEIRO, S. G.; MEHTA, A.; SANCHES, M. M.; FONTES, W.; KITAJIMA, E. W.; CRUZ, F. T.; SANTOS, A. M. C.; VENTURA, J. A.; GOMES, A. C. M. M.; ZERBINI, F. M.; SOSA-ACOSTA, P.; NOGUEIRA, F. C. S.; RODRIGUES, S. P.; ARAGÃO, F. J. L.; WHITFIELD, A. E.; FERNANDES, P. M. B. |
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Afiliação: |
Marlonni Maurastoni, UFES; Tathiana F. Sá Antunes, UFES; Emanuel F. M. Abreu, Embrapa Recursos Genéticos e Biotecnologia; Simone G. Ribeiro, Embrapa Recursos Genéticos e Biotecnologia; Angela Mehta, Embrapa Recursos Genéticos e Biotecnologia; Marcio M. Sanches, Embrapa Recursos Genéticos e Biotecnologia; Wagner Fontes, UNB; Elliot W. Kitajima, USP; Fabiano T. Cruz, UFES; Alexandre M. C. Santos, UFES; Jose Aires Ventura, Incaper; Ana C. M. M. Gomes, Embrapa Recursos Genéticos e Biotecnologia; F. Murilo Zerbini, UFV; Patricia Sosa-Acosta, UFRJ; Fábio C. S. Nogueira, UFRJ; Silas P. Rodrigues, UFRJ; Francisco J. L. Aragão, Embrapa Recursos Genéticos e Biotecnologia; Anna E. Whitfield, North Carolina State University; Patricia M. B. Fernandes, UFES. |
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Título: |
A capsid protein fragment of a Toti-like Virus Found in Carica papaya Latex interacts with the 50S ribosomal protein L17 |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Viruses, v. 15, n. 541, p. 1-20, 2023. |
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DOI: |
10.3390/v15020541 |
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Idioma: |
Inglês |
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Conteúdo: |
Papaya sticky disease is caused by the association of a fusagra-like and an umbra-like virus, named papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), respectively. Both viral genomes are encapsidated in particles formed by the PMeV ORF1 product, which has the potential to encode a protein with 1563 amino acids (aa). However, the structural components of the viral capsid are unknown. To characterize the structural proteins of PMeV and PMeV2, virions were purified from Carica papaya latex. SDS-PAGE analysis of purified virus revealed two major proteins of ~40 kDa and ~55 kDa. Amino-terminal sequencing of the ~55 kDa protein and LC-MS/MS of purified virions indicated that this protein starts at aa 263 of the deduced ORF1 product as a result of either degradation or proteolytic processing. A yeast two-hybrid assay was used to identify Arabidopsis proteins interacting with two PMeV ORF1 product fragments (aa 321?670 and 961?1200). The 50S ribosomal protein L17 (AtRPL17) was identified as potentially associated with modulated translation-related proteins. In plant cells, AtRPL17 co-localized and interacted with the PMeV ORF1 fragments. These findings support the hypothesis that the interaction between PMeV/PMeV2 structural proteins and RPL17 is important for virus?host interactions. |
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Palavras-Chave: |
Meleira. |
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Thesagro: |
Carica Papaya; Mamão; Proteína; Vírus. |
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Categoria do assunto: |
-- |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/item/4389/1/A-Capsid-Protein-Fragment-of-a-Toti-like-Virus-2023.pdf
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Marc: |
LEADER 02456naa a2200409 a 4500
001 1024693
005 2023-04-25
008 2023 bl uuuu u00u1 u #d
024 7 $a10.3390/v15020541$2DOI
100 1 $aMAURASTONI, M.
245 $aA capsid protein fragment of a Toti-like Virus Found in Carica papaya Latex interacts with the 50S ribosomal protein L17$h[electronic resource]
260 $c2023
520 $aPapaya sticky disease is caused by the association of a fusagra-like and an umbra-like virus, named papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), respectively. Both viral genomes are encapsidated in particles formed by the PMeV ORF1 product, which has the potential to encode a protein with 1563 amino acids (aa). However, the structural components of the viral capsid are unknown. To characterize the structural proteins of PMeV and PMeV2, virions were purified from Carica papaya latex. SDS-PAGE analysis of purified virus revealed two major proteins of ~40 kDa and ~55 kDa. Amino-terminal sequencing of the ~55 kDa protein and LC-MS/MS of purified virions indicated that this protein starts at aa 263 of the deduced ORF1 product as a result of either degradation or proteolytic processing. A yeast two-hybrid assay was used to identify Arabidopsis proteins interacting with two PMeV ORF1 product fragments (aa 321?670 and 961?1200). The 50S ribosomal protein L17 (AtRPL17) was identified as potentially associated with modulated translation-related proteins. In plant cells, AtRPL17 co-localized and interacted with the PMeV ORF1 fragments. These findings support the hypothesis that the interaction between PMeV/PMeV2 structural proteins and RPL17 is important for virus?host interactions.
650 $aCarica Papaya
650 $aMamão
650 $aProteína
650 $aVírus
653 $aMeleira
700 1 $aANTUNES, T. F. S.
700 1 $aABREU, E. F. M.
700 1 $aRIBEIRO, S. G.
700 1 $aMEHTA, A.
700 1 $aSANCHES, M. M.
700 1 $aFONTES, W.
700 1 $aKITAJIMA, E. W.
700 1 $aCRUZ, F. T.
700 1 $aSANTOS, A. M. C.
700 1 $aVENTURA, J. A.
700 1 $aGOMES, A. C. M. M.
700 1 $aZERBINI, F. M.
700 1 $aSOSA-ACOSTA, P.
700 1 $aNOGUEIRA, F. C. S.
700 1 $aRODRIGUES, S. P.
700 1 $aARAGÃO, F. J. L.
700 1 $aWHITFIELD, A. E.
700 1 $aFERNANDES, P. M. B.
773 $tViruses$gv. 15, n. 541, p. 1-20, 2023.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
21/09/2021 |
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Data da última atualização: |
23/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SALLES, T. S.; MENESES, M. D. F.; CALDAS, L. A.; SÁ-GUIMARÃES, T. E.; OLIVEIRA, D. M. de; VENTURA, J. A.; AZEVEDO, R. C.; KUSTER, R. M.; SOARES, M. R.; FERREIRA, D. F. |
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Afiliação: |
Tiago Souza Salles; Marcelo Damião Ferreira Meneses; Lucio Ayres Caldas; Thayane Encarnação Sá-Guimarães; Danielle M. de Oliveira; Jose Aires Ventura, Incaper; Renata Campos Azevedo; Ricardo M. Kuster; Márcia Regina Soares; Davis Fernandes Ferreira. |
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Título: |
Virucidal and antiviral activities of pomegranate (Punica granatum) extract against the mosquito-borne Mayaro virus. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Parasites Vectors, v. 14, n. 44, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
The arthropod-borne Mayaro virus (MAYV) causes ?Mayaro fever,? a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. There are currently no licensed drugs against most mosquitotransmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil have been subjected to phytochemical investigation for the identification and isolation of antiviral compounds. In the present study, we explored the antiviral
activity of pomegranate extracts in Vero cells infected with Mayaro virus. |
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Palavras-Chave: |
Biflavonoids; Punicalagin. |
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Thesaurus NAL: |
Alphavirus; Mayaro virus. |
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Categoria do assunto: |
-- |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4239/1/Virucidal-andantiviral-ventura.pdf
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Marc: |
LEADER 01446naa a2200277 a 4500
001 1023713
005 2021-09-23
008 2021 bl uuuu u00u1 u #d
100 1 $aSALLES, T. S.
245 $aVirucidal and antiviral activities of pomegranate (Punica granatum) extract against the mosquito-borne Mayaro virus.$h[electronic resource]
260 $c2021
520 $aThe arthropod-borne Mayaro virus (MAYV) causes ?Mayaro fever,? a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. There are currently no licensed drugs against most mosquitotransmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil have been subjected to phytochemical investigation for the identification and isolation of antiviral compounds. In the present study, we explored the antiviral activity of pomegranate extracts in Vero cells infected with Mayaro virus.
650 $aAlphavirus
650 $aMayaro virus
653 $aBiflavonoids
653 $aPunicalagin
700 1 $aMENESES, M. D. F.
700 1 $aCALDAS, L. A.
700 1 $aSÁ-GUIMARÃES, T. E.
700 1 $aOLIVEIRA, D. M. de
700 1 $aVENTURA, J. A.
700 1 $aAZEVEDO, R. C.
700 1 $aKUSTER, R. M.
700 1 $aSOARES, M. R.
700 1 $aFERREIRA, D. F.
773 $tParasites Vectors$gv. 14, n. 44, 2021.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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