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Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br.
Registro Completo
Biblioteca(s):  Biblioteca Rui Tendinha.
Data corrente:  06/07/2020
Data da última atualização:  06/07/2020
Tipo da produção científica:  Artigo em Periódico Indexado
Autoria:  MOURA, R. D.; CASTRO, L. A. M. de; CULIK, M. P.; FERNANDES, A. A. R.; FERNANDES, P. M. B.; VENTURA, J. A.
Afiliação:  Raíssa Debacker Moura; Luiza Adami Monteiro de Castro; Mark Paul Culik, CNPq/Incaper; Antônio Alberto Ribeiro Fernandes; Patricia Machado Bueno Fernandes; Jose Aires Ventura, Incaper.
Título:  Culture medium for improved production of conidia for identification and systematic studies of Fusarium pathogens.
Ano de publicação:  2020
Fonte/Imprenta:  Journal of Microbiological Methods, v. 173, June 2020.
DOI:  10.1016/j.mimet.2020.105915
Idioma:  Inglês
Conteúdo:  Fusarium guttiforme and Fusarium ananatum are the etiological agents of fusariosis and fruitlet core rot in pineapple, espectively, producing mycotoxins that are harmful to the health of consumers. These two fungi are morphologically similar and difficulty in obtaining macroconidia of the species limits their identification. Different types of media are available for the culture of these pathogens, but not all of them favor F. ananatum and F. guttiforme macroconidia production. Therefore, the objective of this study was to develop a simple culture medium to improve rapid macro- and microconidia formation in both F. guttiforme and F. ananatum to facilitate taxonomic, pathogenicity and mycotoxin studies. In vitro analysis showed that basal medium with carboxymethyl cellulose (CMC) was better than other media tested with the highest macroconidia production at 7 days of incubation. The highest production of microconidia was with synthetic nutrient medium (SN) at 7 days. F. ananatum produced a relatively high number of microconidia with one septum in comparison to F. guttiforme when cultured in CMC, which suggests an additional character useful for Fusarium taxonomy. CMC medium may serve as an improved alternative to culture media currently used in Fusarium research and contribute to further knowledge of the taxonomy and mycotoxins of Fusarium species.
Palavras-Chave:  Abacaxi.
Thesagro:  Fusariose; Fusarium; Patogenicidade; Taxonomia.
Thesaurus NAL:  Carboxymethyl cellulose; Sporulation; Taxonomy.
Categoria do assunto:  H Saúde e Patologia
Marc:  Mostrar Marc Completo
Registro original:  Biblioteca Rui Tendinha (BRT)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status
BRT - MI23443 - 1UMTAP - DD

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Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br.
Registro Completo
Biblioteca(s):  Biblioteca Rui Tendinha.
Data corrente:  17/04/2018
Data da última atualização:  17/04/2018
Tipo da produção científica:  Artigo em Periódico Indexado
Circulação/Nível:  A - 1
Autoria:  MADRONERO, J.; RODRIGUES, S. P.; ANTUNES, T. F. S.; ABREU, P. M. V.; VENTURA, J. A.; FERNANDES, A. A. R.; FERNANDES, P. M. B.
Afiliação:  Johana Madroñero, UFES; Silas P. Rodrigues, UFES; Tathiana F. S. Antunes, UFES; Paolla M. V. Abreu, UFES; Jose Aires Ventura, Incaper; A. Alberto R. Fernandes, UFES; Patricia Machado Bueno Fernandes, UFES.
Título:  Transcriptome analysis provides insights into the delayed sticky disease symptoms in Carica papaya
Ano de publicação:  2018
Fonte/Imprenta:  Plant Cell Reports, p. 1-14, 2018.
Idioma:  Português
Conteúdo:  Carica papaya plants develop the papaya sticky disease (PSD) as a result of the combined infection of papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), or PMeV complex. PSD symptoms appear only after C. papaya flowers. To understand the mechanisms involved in this phenomenon, the global gene expression patterns of PMeV complex-infected C. papaya at pre-and post-flowering stages were assessed by RNA-Seq. The result was 633 and 88 differentially expressed genes at pre- and post-flowering stages, respectively. At pre-flowering stage, genes related to stress and transport were up-regulated while metabolism-related genes were down-regulated. It was observed that induction of several salicylic acid (SA)-activated genes, including PR1, PR2, PR5, WRKY transcription factors, ROS and callose genes, suggesting SA signaling involvement in the delayed symptoms. In fact, pre-flowering C. papaya treated with exogenous SA showed a tendency to decrease the PMeV and PMeV2 loads when compared to control plants. However, pre-flowering C. papaya also accumulated transcripts encoding a NPR1-inhibitor (NPR1-I/NIM1-I) candidate, genes coding for UDP-glucosyltransferases (UGTs) and several genes involved with ethylene pathway, known to be negative regulators of SA signaling. At post-flowering, when PSD symptoms appeared, the down-regulation of PR-1 encoding gene and the induction of BSMT1 and JA metabolism-related genes were observed. Hence, SA signaling likely operates at the pre-flow... Mostrar Tudo
Thesaurus NAL:  Carica papaya; Defense responses; Papaya meleira virus; Transcriptome Plant'virus interaction.
Categoria do assunto:  --
Marc:  Mostrar Marc Completo
Registro original:  Biblioteca Rui Tendinha (BRT)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status
BRT - MI21087 - 1UMTAP - DD
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