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| 261. |  | PFENNING, L. H.; COSTA, S. da S.; MELO, M. P. de.; COSTA, H.; VENTURA, J. A.; AUER, C. G.; SANTOS, A. F. dos. First report and characterization of Fusarium circinatum, the causal agent of pitch canker in Brazil. Tropical Plant Pathology, vol. 39(3):210-216, 2014.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 262. | | FONSECA-BOITEUX, M. E. de N.; BOITEUX, L. S.; LIMA, M. F.; MENDONCA, J. L. de; COSTA, A. F.; FONTES, M. G.; COSTA, H.; GONZÁLEZ-ARCOS, M. First report of tomato chlorosis vírus infecting eggplant and scarlet eggplant in Brazil. Plant disease, v. 100, n. 4, p. 867, 2016. On line.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 263. | | LIMA, I. de M.; VENTURA, J. A.; COSTA, H.; SILVA, I. R.; SANTOS, E. P. dos; ROCHA, T. T. T. da Fitonematoides : coleta e envio de amostras nematológicas. Vitória, ES : Incaper, 2022. (Incaper, Documentos, 294). Folder.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 264. | | LIMA, R. de C. A.; COUTO, A. de O. F.; ANDRADE, J. de S.; MARTINS, D. dos S.; VENTURA, J. A.; COSTA, H. Flutuação populacional de insetos vetores de doença do mamoeiro e sua relação com a ocorrência de doenças viróticas. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 1., 2003, Vitória. MARTINS, D. dos S. (Ed.). Papaya Brasil : qualidade do mamão para o mercado interno. Vitória : Incaper, 2003. p. 539-541.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 265. | | FERRÃO, M. A. G.; FONSECA, A. F. A. da.; FERRÃO, R. G.; BARBOSA, W. M.; COSTA, H.; SAKAYAMA, N. S.; VOLPI, P. S. Identificação de marcadores ligados a genes de resistência à ferrugem em coffea canephora variedade conilon, através de RPD e microssatélite, e caracterização molecular de genótipos da espécie. In: SIMPÓSIO INTERNO DE PESQUISA, DESENVOLVIMENTO E INOVAÇÃO, 1., 2004, Vitória. Resumo das ações de pesquisa, desenvolvimento e inovações tecnológicas. Vitória, ES : Incaper, p. 63-64, 2005. (Incaper. Documentos, 140).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 266. | | COSTA, H.; ZAMBOLIM, L.; VENTURA, J. A.; VALE, F. X. R. do.; ARAÚJO, G. A. A. de.; SILVA, M. B. da. Identificação de raças de fusarium oxysporum f. sp. phaseoli. REUNIAO NACIONAL DE PESQUISA DE FEIJAO, 6, Goiânia, v.1, p.194-197, 1999. Anais... Goiania, GO: EBDA, EMBRAPA, 1999.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 268. | | FORNAZIER, M. J.; COSTA, H.; ROCHA, A. C. da.; MORELI, A. P.; FERRÃO, M. A. G.; FERRÃO, R. G.; FONSECA, A. F. A. da. Incidência de pragas e doenças em talhões de café arábica e conilon em propriedade certificada - UTZ CERTIFIED - na região das montanhas do Espírito Santo. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 35., 2009, Araxá. Trabalhos apresentados... Varginha, MG: Fundação PROCAFÉ, 2009.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 270. | | LIMA, I. de M.; VENTURA, J. A.; COSTA, H.; VOLPI, P. S.; MAURI, A. L.; ANDRADE JUNIOR, S.; PONTE MELO, E. C. Ocorrência de Meloidogyne incognita em cafeeiro robusta variedade Bukobensis no município de Marilândia/Espírito Santo. Tropical Plant Pathology, Brasília, DF, v. 35, p. S224, ago. 2010. Suplemento, ref. 07.104. Edição dos resumos do XLIII Congresso Brasileiro de Fitopatologia, Cuiabá, ago. 2010.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 271. | | ÁVILA, A. C. de; INOUE-NAGATA, A. K.; COSTA, H.; BOITEUX, L. S.; NEVES, L. O. de; PRATES, R. S.; BERTINI, L. A. Ocorrência de viroses em tomate e pimentão na região serrana do Estado do Espírito Santo. Horticultura Brasileira, Brasília, DF: v. 22, n. 3, p. 655-658, jul./set. 2004.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 272. | | ALVES, F. de L.; CUNHA SOBRINHO, A. P. da.; POMPEU JUNIOR, J.; COSTA, H.; FULLIN, E. A.; FORNAZIER, M. J.; COSTA, W. Produção de borbulhas selecionadas de Citrus no Estado do Espírito Santo. Vitória, ES: Emcapa, 1992. (EMCAPA. Documentos, 75).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 273. | | ALVES, F. de L.; CUNHA SOBRINHO, A. P. da; POMPEU JUNIOR, J.; COSTA, H.; FULLIN, E. A.; FORNAZIER, M. J.; COSTA, W. Producao de borbulhas selecionadas de Citrus spp. pelo sistema de borbulheira no Estado do Espirito Santo Revista Brasileira de Fruticultura, Cruz das Almas, v.14, n.2, p.229-233, 1992.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 274. | | ROCHA, A. C. da.; FONSECA, A. F. A. da.; FERRÃO, M. A. G.; FORNAZIER, M. J.; COSTA, H.; PREZOTTI, L. C.; BOREL, R. M. A. Produção e qualidade do café arábica no contexto de agricultores familiares do Estado do Espírito Santo. In: SIMPÓSIO INTERNO DE PESQUISA, DESENVOLVIMENTO E INOVAÇÃO, 1., 2004, Vitória. Resumo das ações de pesquisa, desenvolvimento e inovações tecnológicas. Vitória, ES : Incaper, p. 53-54, 2005. (Incaper. Documentos, 140).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 275. | | FORNAZIER, M. J.; COSTA, H.; ROCHA, A. C. da.; MORELI, A. P.; FERRÃO, M. A. G.; FERRÃO, R. G.; FONSECA, A. F. A. da.; BAUTZ, A. Monitoramento de bicho mineiro, ferrugem, cercóspora e phoma em cafés arábica e conilon em propriedade Utz Certified, Montanhas do Espírito Santo. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 36., 2010, Guarapari. Trabalhos apresentados... Brasília, DF: MAPA/PROCAFÉ: Embrapa Café; Lavras: UFLA; Uberaba: UNIUBE; Varginha: Fundação Procafé; Vitória: INCAPER, p. 246-248, 2010.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 276. | | COSTA, H. B.; SOUZA, L. M.; SOPRANI, L. C.; OLIVEIRA, B. G.; OGAWA, E. M.; KORRES, A. M. N.; VENTURA, J. A.; ROMÃO, W. Monitoring the physicochemical degradation of coconut water using ESI-FT-ICR MS. Food Chemistry, Volume 174, 1 May 2015, Pages 139-146.| Biblioteca(s): Biblioteca Rui Tendinha. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
09/09/2020 |
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Data da última atualização: |
09/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DUARTE, M. F.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; ROJAS, M. J.; GILBERTSON, R.; COSTA, H.; BOITEUX, L. S.; FONSECA, M. E. N. |
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Afiliação: |
Macaria Ferreira Duarte, UnB; Rita de Cássia Pereira Carvalho, UnB; Luciane de Nazaré Almeida dos Reis, UnB; Maria J. Rojas, UC-DAVIS; Robert Gilbertson, UC-DAVIS; Helcio Costa, Incaper; Leonardo Silva Boiteux, EMBRAPA; Maria Esther N. Fonseca, EMBRAPA. |
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Título: |
Natural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Disease, set. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Severe yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. MenosSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia ... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Erva Daninha; Mosaico; Tomate. |
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Thesaurus NAL: |
Begomovirus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4073/1/natural-infection-tomatoes-costa.pdf
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Marc: |
LEADER 04125naa a2200265 a 4500
001 1022277
005 2020-09-09
008 2020 bl uuuu u00u1 u #d
100 1 $aDUARTE, M. F.
245 $aNatural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States.$h[electronic resource]
260 $c2020
520 $aSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop.
650 $aBegomovirus
650 $aDoença de Planta
650 $aErva Daninha
650 $aMosaico
650 $aTomate
700 1 $aPEREIRA-CARVALHO, R. de C.
700 1 $aREIS, L. de N. A. dos
700 1 $aROJAS, M. J.
700 1 $aGILBERTSON, R.
700 1 $aCOSTA, H.
700 1 $aBOITEUX, L. S.
700 1 $aFONSECA, M. E. N.
773 $tPlant Disease, set. 2020.
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