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| Registros recuperados : 65 | |
| 23. |  | SILVA, W. P. da; QUADROS, O. de F.; FERNANDES, P. M. B.; VENTURA, J. A. Pré-aclimatação de plantas transformadas por CRISPR/CAS9 para deleção do gene ?-1,3-glucanase e por gateway para superexpressão da proteína Quitinase em Carica Papaya L. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 8. , Linhares, ES. Papaya Brasil : produção e sustentabilidade. Organizadores, David dos Santos Martins e José Aires Ventura. Vitória, ES : Incaper, 2022. Anais... Linhares, ES : Incaper, p. 204-209, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 25. | | PINHEIRO, E. B.; VENTURA, J. A.; COSTA, H.; FERNANDES, P. M. B. O uso do banco de dados Agroimpacto Mamão na produção integrada de mamão. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 3. , 2007, Vitória. Anais... Vitória: CEDAGRO; INCAPER, 2007. Papaya Brasil : manejo, qualidade e mercado do mamão. Vitória: Incaper, 2007. p. 441-444| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 26. | | ANDRADE, J. de S.; VENTURA, J. A.; RODRIGUES, S. P.; FERNANDES, P. M. B.; TATAGIBA, J. da S.; COSTA, H. Avaliação de diferentes métodos de inoculação da meleira em plantas jovens de mamão. Fitopatologia Brasileira, Fortaleza, v.28, n. Supl., p.288, 2003. Resumo.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 27. | | ARAUJO, M. M.; VAZ, A. B.; BORGES, B. J. P.; VENTURA, J. A.; FERNANDES, P. M. B. Biossegurança na propagação de plantas geneticamente modificadas : um olhar da legislação brasileira sobre a fitossanidade. In: ENCONTRO BIENAL CAPIXABA DE BIOSEGURANÇA, 7.; ENCONTRO BIENAL RENORBIO, 4, 2016, Vitória. Biotecnologia Industrial. Vitória, ES : UFES, 2016.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 28. | | ALMEIDA, M. de A.; ARAÚJO, M. M.; VENTURA, J. A.; SANTOS, A. M. C.; FERNANDES, P. M. B. Análise estrutural in silico de um polipeptídeo da proteína capsidial do papaya meleira vírus. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 8. , Linhares, ES. Papaya Brasil : produção e sustentabilidade. Organizadores, David dos Santos Martins e José Aires Ventura. Vitória, ES : Incaper, 2022. Anais... Linhares, ES : Incaper, p. 216-221, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 31. | | SANTOS, M. P.; ALVES, E. S. S.; SANTOS, R. B.; VENTURA, J. A.; FERNANDES, P. M. B. Controle de Colletotrichum gloeosporioides agente etiológico da antracnose do mamão por óleos essenciais. Fitopatologia Brasileira, Brasília, DF, v. 27, p. S162, 2002. Suplemento. Resumo. Apresentado no 35º Congresso Brasileiro de Fitopatologia, 2002. Resumo.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 33. | | ALVES, E. S. S.; SANTOS, M. P.; SANTOS, R. B.; VENTURA, J. A.; FERNANDES, P. M. B. Eficiência de óleos essenciais no controle "in vitro' da germinação de conídios e do crescimento micelial de Colletotrichum musae. Fitopatologia Brasileira, Brasília, DF, v. 27, p. S75, 2002. Suplemento. Resumo. Apresentado no 35º Congresso Brasileiro de Fitopatologia, 2002. Resumo.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 35. | | VENTURA, J. A.; COSTA, H.; PTENNING, L. H.; SALGADO, J. S.; FERNANDES, P. M. B.; FIRAVANTE, R. L. Dinâmica do desenvolvimento de fungos toxicogênicos e da produção de Ocratoxina A em frutos de café, planta e solo no Estado do Espírito Santo. In: SIMPÓSIO INTERNO DE PESQUISA, DESENVOLVIMENTO E INOVAÇÃO, 1., 2004, Vitória. Resumo das ações de pesquisa, desenvolvimento e inovações tecnológicas. Vitória, ES : Incaper, p. 57-58, 2005. (Incaper. Documentos, 140).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 36. | | PALHANO, F. L.; VILCHES, T. T. B.; SANTOS, R. B.; ORLANDO, M. T. D.; FERNANDES, P. M. B. Inativação de esporos de Colletotrichum gloeosporioides por ação combinada do citral ou óleo essencial de capim-limão e alta pressão hidrostática. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 1., 2003, Vitória. MARTINS, D. dos S. (Ed.). Papaya Brasil : qualidade do mamão para o mercado interno. Vitória : Incaper, 2003. p. 587-590.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 39. | | RODRIGUES,S. P.; PICCIN, J. G. Z.; VALE, P. M.; VENTURA, J. A.; FERNANDES, P. M. B. Papaya meleira virus (PMeV) a a meleira do mamoeiro : estudos realizados no Núcleo de Biotecnologia da UFES. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 4., 2009, VITÓRIA-ES. Pesquisa científica e a crise no mercado do mamão: anais... Vitória: Incaper, 2009. 6p. (Incaper. Documentos, 176). Palestra.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| Registros recuperados : 65 | |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
25/09/2014 |
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Data da última atualização: |
01/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ABREU, P. M. V.; GASPAR, C. G.; BUSS, D. S.; VENTURA, J. A.; FERREIRA, P. C. G.; FERNANDES, P. M. B. |
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Afiliação: |
Paolla M. V. Abreu, Universidade Federal do Espíríto Santo; Clicia G. Gaspar, Univervidade Federal do Rio de Janeiro; David S. Buss, Universidade Federal do Espírito Santo; Jose Aires Ventura, Incaper; Paulo C. G. Ferreira, Universidade Federal do Rio de Janeiro; Patricia M. B. Fernandes, Universidade Federal do Espírito Santo. |
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Título: |
Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
PLOS ONE, v. 9, issue 7, p. 1-13, july 2014. |
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Páginas: |
13 p. |
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Descrição Física: |
il., color. |
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ISSN: |
1932-6203 |
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DOI: |
10.1371 |
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Idioma: |
Português |
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Conteúdo: |
MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. MenosMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for thi... Mostrar Tudo |
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Palavras-Chave: |
Carica papaya; Doenças; Mamão; Meleira; MiRNAs; PMeV; Vírus. |
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Thesaurus NAL: |
Carica papaya; Disease; Meleira; MiRNAs; PMeV; Virus. |
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Categoria do assunto: |
-- |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/397/1/Carica-papaya-microRNAS-Are-responsive-to-papaya.pdf
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Marc: |
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245 $aCarica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection.$h[electronic resource]
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300 $a13 p.$cil., color.
520 $aMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya.
650 $aCarica papaya
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