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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
05/07/2020 |
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Data da última atualização: |
05/07/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAURASTONI, M.; SÁ-ANTUNES, T. F.; OLIVEIRA, S.; SANTOS, A. M. C.; VENTURA, J. A.; FERNANDES, P. M. B. |
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Afiliação: |
Marlonni Maurastoni, UFES; Tathiana F. Sá?Antunes, UFES; Scarlett A. Oliveira, UFES; Alexandre M. C. Santos, UFES; Jose Aires Ventura, Incaper; Patricia M. B. Fernandes, UFES. |
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Título: |
A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Archives of Virology, v. 165, p. 1211-1214, july 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction. |
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Palavras-Chave: |
Doença das Planta; Multiplex; Papaya meleira virus (PMeV). |
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Thesagro: |
Carica papaya; Doença de planta. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01439naa a2200241 a 4500
001 1022233
005 2020-07-05
008 2020 bl uuuu u00u1 u #d
100 1 $aMAURASTONI, M.
245 $aA multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants.$h[electronic resource]
260 $c2020
520 $aPapaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.
650 $aCarica papaya
650 $aDoença de planta
653 $aDoença das Planta
653 $aMultiplex
653 $aPapaya meleira virus (PMeV)
700 1 $aSÁ-ANTUNES, T. F.
700 1 $aOLIVEIRA, S.
700 1 $aSANTOS, A. M. C.
700 1 $aVENTURA, J. A.
700 1 $aFERNANDES, P. M. B.
773 $tArchives of Virology$gv. 165, p. 1211-1214, july 2020.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
10/04/2014 |
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Data da última atualização: |
10/04/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MENEGUZZI, V. C.; SANTOS, C. B. dos; PINTO, I. de S.; FEITOZA, L. R.; FEITOZA, H. N.; FALQUETO, A. |
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Afiliação: |
Viviane Coutinho Meneguzzi, Universidade Federal do Espírito Santo; Claudiney Biral dos Santos, Ministério da Saúde; Israel de Souza Pinto, Instituto Oswaldo Cruz; Leandro Roberto Feitoza, Incaper; Hideko Nagatani Feitoza, Incaper; Aloísio Falqueto, Universidade Federal do Espírito Santo. |
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Título: |
Use of geoprocessing to define malaria risk areas and evaluation of the vectorial importance of anopheline mosquitoes (Diptera : Culicidae) in Espírito Santo, Brazil. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104 (4) : 570-575, jul. 2009. |
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Páginas: |
6 p. |
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Descrição Física: |
il. |
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ISSN: |
1678-8060 |
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Idioma: |
Inglês |
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Conteúdo: |
In Brazil, introduced malaria occurs from the flat to the sloping hot areas, predominantly outside the Amazon Region, where endemic malaria has occurred in the past. This is a consequence of human migrations to other Brazilian states, including the state of Espírito Santo (ES). The objective of this study was to use geoprocessing to define the areas at risk of introduced malaria transmission and evaluate the vectorial importance of species of anophelines in ES. Anophelines were sampled from 1997-2005 in 297 rural localities identified or not identified as foci of malaria during the last 20 years. The geoclimatic variables temperature, relief and marine influence were obtained from a database of the ES Natural Units. The 14,663 anophelines captured belonged to 22 species. A significant association was found between the occurrence of malaria foci and the presence of hot, low-lying areas or gently undulating to undulating relief. The occurrence of the disease was associated with the presence of Anopheles darlingi and Anopheles aquasalis. Geoprocessing was determined to be a useful tool for defining areas at risk for malaria and vectors in ES. |
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Palavras-Chave: |
Anopheles; Brazil; Espírito Santo; Geoclimatic variables; Malaria. |
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Categoria do assunto: |
-- |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/346/1/104-4-916.pdf
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Marc: |
LEADER 02011naa a2200265 a 4500
001 1002778
005 2014-04-10
008 2009 bl --- 0-- u #d
022 $a1678-8060
100 1 $aMENEGUZZI, V. C.
245 $aUse of geoprocessing to define malaria risk areas and evaluation of the vectorial importance of anopheline mosquitoes (Diptera$bCulicidae) in Espírito Santo, Brazil.$h[electronic resource]
260 $c2009
300 $a6 p.$cil.
520 $aIn Brazil, introduced malaria occurs from the flat to the sloping hot areas, predominantly outside the Amazon Region, where endemic malaria has occurred in the past. This is a consequence of human migrations to other Brazilian states, including the state of Espírito Santo (ES). The objective of this study was to use geoprocessing to define the areas at risk of introduced malaria transmission and evaluate the vectorial importance of species of anophelines in ES. Anophelines were sampled from 1997-2005 in 297 rural localities identified or not identified as foci of malaria during the last 20 years. The geoclimatic variables temperature, relief and marine influence were obtained from a database of the ES Natural Units. The 14,663 anophelines captured belonged to 22 species. A significant association was found between the occurrence of malaria foci and the presence of hot, low-lying areas or gently undulating to undulating relief. The occurrence of the disease was associated with the presence of Anopheles darlingi and Anopheles aquasalis. Geoprocessing was determined to be a useful tool for defining areas at risk for malaria and vectors in ES.
653 $aAnopheles
653 $aBrazil
653 $aEspírito Santo
653 $aGeoclimatic variables
653 $aMalaria
700 1 $aSANTOS, C. B. dos
700 1 $aPINTO, I. de S.
700 1 $aFEITOZA, L. R.
700 1 $aFEITOZA, H. N.
700 1 $aFALQUETO, A.
773 $tMemórias do Instituto Oswaldo Cruz, Rio de Janeiro$gv. 104 (4) : 570-575, jul. 2009.
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